文献支持

beta Actin antibody

价格￥1700 - 4000

品牌GeneTex
产地美国
货号
GTX109639
更新日期2025年07月16日

应用范围WB, ICC/IF, IHC-P, IHC-Fr, IP
宿主Rabbit
适应物种Human, Mouse, Rat, Yeast, Sheep, Drosophila, Cat, Dog, Hamster, Chicken, Pig, Monkey, Caenorhabditis elegans, Candida albicans, E. coli, Horse, Rice, Xenopus, Treeshrew, Plant, Worm

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详细信息
询价记录
文献和实验
技术资料

免疫原：
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.
亚型：
IgG
形态：
Liquid
保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性：
Polyclonal
标记物：
Unconjugated
适应物种：
Human, Mouse, Rat, Yeast, Sheep, Drosophila, Cat, Dog, Hamster, Chicken, Pig, Monkey, Caenorhabditis elegans, Candida albicans, E. coli, Horse, Rice, Xenopus, Treeshrew, Plant, Worm
保质期：
12 months from the shipping date of the product.
抗原来源：
Human
目录编号：
GTX109639
级别：
Primary Antibodies
库存：
Available
供应商：
GeneTex
宿主：
Rabbit
应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, IP
浓度：
1.4 mg/ml (Please refer to the vial label for the specific concentration.)
靶点：
IP/MS validation was supported by references (PMID:30377395)
抗体英文名：
beta Actin antibody
抗体名：
beta Actin 抗体
规格：
100 μl/25 μl

规格：	100 μl	产品价格：	￥4000.0
规格：	25 μl	产品价格：	￥1700.0

Sample (30 μg of whole cell lysate)
A: 293T
B: NTH-3T3
C: mouse brain
D: PC-12
E: Rat brain
F: drosophila
10% SDS PAGE
GTX109639 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
beta Actin stained by beta Actin antibody (GTX109639) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (30 μg of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
10% SDS PAGE
GTX109639 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

beta Actin antibody detects beta Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
beta Actin stained by beta Actin antibody (GTX109639) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta Actin stained by beta Actin antibody (GTX109639) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (whole cell lysate)
A: Candida albicans (sc5314) 20ug
B: Candida albicans (sc5314) 40ug
10% SDS PAGE
GTX109639 diluted at 1:4000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

beta Actin antibody detects ACTB protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
10% SDS-PAGE
beta Actin antibody (GTX109639) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX109639) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin stained by beta Actin antibody (GTX109639) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX109639) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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