- ￥2062
- LSM Bio
- 进口
- PAab00869
- 2025年07月17日
- ELISA,IF,WB,IHC
- E. coli - derived recombinant protein
- Human,Mouse ,Rat,Zebrafish, Monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
actin, beta
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
Polyclonal antibody
- 标记物：
Non-conjugated
- 适应物种：
Human,Mouse ,Rat,Zebrafish, Monkey
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
Q9GZT9
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA,IF,WB,IHC
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
beta actin
- 抗体英文名：
anti-beta actin antibody,beta actin antibody
- 抗体名：
anti-beta actin 抗体,beta actin 抗体
- 规格：
100μg
beta actin抗体| beta actin antibody
货号 PAab00869
蛋白别名 ACTB antibody, Actin antibody, actin antibody, beta antibody, Actin antibody, cytoplasmic 1 antibody, B actin antibody, Beta actin antibody, F actin antibody, β actin
蛋白介绍
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins.
产品描述
anti-beta actin antibody is a Rabbit Polyclonal antibody againstbeta actin..
建议稀释比例
WB:1:1000-1:10000;IHC:1:20-1:500;IF:1:10-1:100
IHC
Immunohistochemistry of paraffin-embedded human heart using PAab00869(ACTB antibody) at dilution of 1:100
Western blot
C6 cells were subjected to SDS PAGE followed by western blot with PAab00869( beta actin Antibody) at dilution of 1:2000(本抗体仅供体外科研用途，不可用于临床诊断!)
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文献和实验beta actin is a type of cell skeleton protein.Its gene is a house keeping gene.In most of cells，beta actin，such as human skeleton，is expressed in a certain level.THat is the reason that people use beta-actin as the criteria of cell numbers
【求助】细胞里的beta catenin 好检测吗？？ 用 western blot
antonio2001 细胞里的beta catenin 好检测吗？？ 用 western blot 和免疫细胞化学 ？？ 2008851029 细胞里的beta-actin非常好检测（我用的是ST的抗体） littlebee83325 什么细胞呢？胞浆蛋白么？ magichunter beta catenin 好检测，细胞全蛋白裂解液上样20uL（15
familar 小妹困惑，在此请教大师！ 做了几次beta-catenin的表达（western），都没做出来，目的蛋白的分子量是92，可是，做出来的印记总是在下方，明显的不是目的蛋白的位置。我师兄说，做beta-catenin要提核蛋白才能做出来，可我之前也有做出来过的！请教各位有经验的同志，这样的现象是何解？ woxingwosu 不一定要提核蛋白才能出来。b-catenin在核内核外都有分布。应该
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