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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human MCM7. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX110278
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IP, ChIP assay
- 浓度:
0.76 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
IP/MS validation was supported by references (PMID:30377399)
- 抗体英文名:
MCM7 antibody [N2C2], Internal
- 抗体名:
MCM7 抗体 [N2C2], Internal
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using MCM7(GTX110278) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (30 μg of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX110278 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunofluorescence analysis of PFA-fixed HeLa, using MCM7(GTX110278) antibody at 1:200 dilution.
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 40 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM7 protein by 4 μg MCM7 antibody [N2C2], Internal (GTX110278)
7.5 % SDS-PAGE
The immunoprecipitated MCM7 protein was detected by MCM7 antibody [N2C2], Internal (GTX110278) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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文献和实验Sikorski K et al., Nat Methods 2018 (PMID:30377371)
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