- ￥1700 - 4000
- GeneTex
- 美国
- GTX127343
- 2025年07月10日
- WB, IHC-Wm
- Rabbit
- Zebrafish, Nothobranchius furzeri
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of zebrafish Histone H2A.X. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Zebrafish, Nothobranchius furzeri
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Zebrafish
- 目录编号：
GTX127343
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-Wm
- 浓度：
1 mg/ml(Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H2A.X
- 抗体英文名：
Histone H2A.X antibody
- 抗体名：
Histone H2A.X 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Histone H2A.X antibody detects Histone H2A.X protein by Western blot analysis. Un-treated (-) and treated (+, 8000J/m2 UVC treatment) 3dpf zebrafish embryos whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX127343) at a dilution of 1:1000.
Histone H2A.X antibody detects Histone H2A.X protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Histone H2A.X antibody (GTX127343) dilution: 1:100.
Zebrafish embryos extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX127343) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Histone H2A.X antibody detects Histone H2A.X protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Histone H2A.X antibody (GTX127343) dilution: 1:100.
H2afx antibody detects H2afx protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: PFA-fixed zebrafish embryos.
H2afx antibody (GTX127343) dilution: 1:200.
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文献和实验Cencioni C et al., Cells 2019 (PMID:31569376)
Histone Acetylation and Deacetylation
transcription (1 ,2 ). DNA is packaged into chromatin, a highly organized and dynamic protein-DNA complex. The fundamental subunit of chromatin, the nucleosome, is composed of an octomer of four core histones, an H3/H4 tetramer and two H2A/H2B dimers, surrounded
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
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