- ￥1700 - 4000
- GeneTex
- 美国
- GTX103071
- 2025年07月08日
- WB, ICC/IF, IP
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the N-terminus region of human MCM2. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX103071
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IP
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
IP/MS validation was supported by references (PMID:30377385)
- 抗体英文名：
MCM2 antibody [N1N2], N-term
- 抗体名：
MCM2 抗体 [N1N2], N-term
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
MCM2 antibody [N1N2], N-term detects MCM2 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% PFA at RT for 15 min.
Green: MCM2 protein stained by MCM2 antibody [N1N2], N-term (GTX103071) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Sample (30 ug of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX103071 diluted at 1:1000
MCM2 antibody [N1N2], N-term immunoprecipitates MCM2 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. 40 μg HCT-116 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM2 protein by 4 μg MCM2 antibody [N1N2], N-term (GTX103071)
7.5 % SDS-PAGE
The immunoprecipitated MCM2 protein was detected by MCM2 antibody [N1N2], N-term (GTX103071) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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文献和实验Sikorski K et al., Nat Methods 2018 (PMID:30377371)
Cheung CHY et al., Sci Rep 2017 (PMID:29038488)
In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
to protect the antibody. These should be stored in smaller aliquots (about 1 ml) and frozen for long term storage though the protected solution is also stable for months at four degrees. Ascites fluid: This is a bit more problematic as it may contain
Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust
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