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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bax. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX109683
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, IP
- 浓度:
1.15 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Bax
- 抗体英文名:
Bax antibody [N1N2], N-term
- 抗体名:
Bax 抗体 [N1N2], N-term
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Bax antibody [N1N2], N-term detects Bax protein at cytoplasm, nucleus and mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Bax protein stained by Bax antibody [N1N2], N-term (GTX109683) diluted at 1:400.
Blue: Hoechst 33342 staining.
Bax antibody [N1N2], N-term detects Bax protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Bax antibody [N1N2], N-term (GTX109683) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Bax antibody [N1N2], N-term immunoprecipitates BCL2-associated X protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of BCL2-associated X protein by 4 μg Bax antibody [N1N2], N-term (GTX109683)
15 % SDS-PAGE
The immunoprecipitated BCL2-associated X protein was detected by Bax antibody [N1N2], N-term (GTX109683) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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