- ￥1700 - 4000
- GeneTex
- 美国
- GTX102111
- 2025年07月11日
- WB, ICC/IF, IHC-P, IHC-Fr, IP, LCI
- Rabbit
- Human, Rat, Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human CD44. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Rat, Rabbit
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX102111
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, IP, LCI
- 浓度：
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
IP/MS validation was supported by references (PMID:30377381)
- 抗体英文名：
CD44 antibody
- 抗体名：
CD44 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Non-transfected (–) and transfected (+) Wild-type (WT) and G CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD44 antibody (GTX102111) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using CD44(GTX102111) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
CD44 antibody detects CD44 protein at membrane and cytoplasm on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma .
CD44 antibody (GTX102111) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded Human pancreatic tumor, using CD44(GTX102111) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
CD44 antibody detects CD44 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
CD44 stained by CD44 antibody (GTX102111) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Chang CC et al., Sci Rep 2018 (PMID:29955155)
Sun SJ et al., Oncotarget 2016 (PMID:27487131)
Aoyagi T et al., JCT 2018 9()
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Ni YL et al., Apoptosis 2017 (PMID:28741092)
Tay LX et al., Int J Med Sci 2014 (PMID:24396283)
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Wang AM et al., Oncotarget 2014 (PMID:24970812)
Isayev O et al., Oncotarget 2014 (PMID:25015789)
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Huang D et al., Mol Pharm 2017 (PMID:27997199)
Lin CW et al., Acta Biomater 2016 (PMID:26853764)
Ju SY et al., Cell Death Dis 2015 (PMID:26136074)
N Yokdang et al., Oncogene 2015 (PMID:26387542)
Liao CJ et al., BMC Cancer 2014 (PMID:25081282)
Jin L et al., Animal Cells and Systems 2014 (Epub)
Ma L et al., Biochem Biophys Res Commun 2014 (Epub)
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Chung SY et al., J Cell Physiol 2019 (PMID:31219187)
LaRocca TJ et al., Neurobiol Dis 2019 (PMID:31229690)
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Nan JN et al., Korean J Intern Med 2018 (PMID:30360026)
Sikorski K et al., Nat Methods 2018 (PMID:30377371)
Lin YH et al., Endocr Relat Cancer 2018 (PMID:30400024)
Chang YT et al., Sci Rep 2017 (PMID:28256535)
CD44/CD44R 分子 CD44 常用单克隆抗体或代号： GRHL1,Hermes,F10- 44- 2(Pgp- 1H- CAM,ECM- RⅢ) 主要表达细胞： Leu,上皮,Fb,RBC[AS] 分子质量(kDa)和结构： gp80- 95 功 能： 粘附ECM,T细胞活化,淋巴细胞归位受体,归位到HEV CD44R 常用单克隆抗体或代号： FM11,24
对CD44的研究时间较长、较深入亦较广泛。已知其为分布极为广泛的细胞表面跨膜糖蛋白，主要参与异质性粘附，即肿瘤细胞与宿主细胞和宿主基质的粘附，异质性粘附在肿瘤细胞侵袭转移中起促进作用。 人类CD44基因位于11号染色体短臂，全长约50kb，共由20个高度保守的外显子组成，每个外显子长度70-210bp不等，中间由长短不一的内含子分隔。CD44基因的20个外显子按照转录方式不同可分为组成型外显子（C）和变异型拼接外显子（V）两大类。组成型外显子共有10个，存在
PCR Analysis of CD44 Variants in Tumors
The CD44 molecule is a transmembrane glycoprotein, it is an acidic, sul fated protein that contains multiple phosphoserine residues and several intrachain disulfide bonds. It is essentially composed of a 37-kDa protein core highly glycosylated
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