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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human TRAP1. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX102017
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, PLA
- 浓度:
0.66 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
TRAP1
- 抗体英文名:
TRAP1 antibody
- 抗体名:
TRAP1 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody (GTX102017) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TRAP1 antibody detects TRAP1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: TRAP1 protein stained by TRAP1 antibody (GTX102017) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
B:MOLT4(GTX27912)
7.5% SDS PAGE
GTX102017 diluted at 1:1500
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody (GTX102017) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TRAP1 antibody detects TRAP1 protein at mitochondrion on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
TRAP1 antibody (GTX102017) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验Lanzillotta C et al., Prog Neurobiol 2020 (PMID:32795489)
Zhao X et al., Sci Rep 2017 (PMID:28397817)
Brauers E et al., Proteomics Clin Appl 2016 (PMID:27739254)
Matassa DS et al., Cancer Cell Int 2022 (PMID:36510251)
Avolio R et al., bioRxiv 2023 (PMID:36712063)
Jia-Huang Chen et al., J Biomed Sci 2022 (PMID:35765067)
Yool Lee et al., Sci Adv 2021 (PMID:33579708)
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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