- ￥1700 - 4000
- GeneTex
- 美国
- GTX101105
- 2025年07月14日
- WB, ICC/IF, IHC-P, IP
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human Prohibitin. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX101105
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
0.34 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Prohibitin
- 抗体英文名：
Prohibitin antibody
- 抗体名：
Prohibitin 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Prohibitin antibody (GTX101105) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (30 μg of whole cell lysate)
A:NIH-3T3
12% SDS PAGE
GTX101105 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Prohibitin(GTX101105) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Prohibitin antibody detects Prohibitin protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Prohibitin protein stained by Prohibitin antibody (GTX101105) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Prohibitin(GTX101105) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Immunoprecipitation of Prohibitin protein from 293T whole cell extracts using 5 μg of Prohibitin antibody (GTX101105).
Western blot analysis was performed using Prohibitin antibody (GTX101105).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Prohibitin antibody (GTX101105) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Prohibitin antibody detects PHB protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
Prohibitin antibody (GTX101105) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Prohibitin antibody detects Prohibitin protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Prohibitin antibody (GTX101105) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Prohibitin antibody detects Prohibitin protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Prohibitin antibody (GTX101105) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Prohibitin antibody detects Prohibitin protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Prohibitin stained by Prohibitin antibody (GTX101105) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Chiu YC et al., Biochem Biophys Rep 2021 (PMID:34786493)
Fang CH et al., J Biomed Sci 2020 (PMID:32169072)
Heng-Hsiung Wu et al., Journal of Food and Drug Analysis 2019 (Epub)
Moriggi M et al., Proteomics 2017 (PMID:29027377)
Yurugi H et al., Oncogene 2017 (PMID:28414306)
Chou HC et al., Arch Biochem Biophys 2014 (PMID:24384558)
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