- ￥1700 - 4000
- GeneTex
- 美国
- GTX100398
- 2025年07月11日
- WB, ICC/IF, IHC-P, IP
- Rabbit
- Human, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the C-terminus region of human DNA polymerase gamma. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX100398
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
0.67 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
DNA polymerase gamma
- 抗体英文名：
DNA polymerase gamma antibody
- 抗体名：
DNA polymerase gamma 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Immunoprecipitation of DNA polymerase gamma protein from MCF-7 whole cell extracts using 5 μg of DNA polymerase gamma antibody (GTX100398).
Western blot analysis was performed using DNA polymerase gamma antibody (GTX100398).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using DNA polymerase gamma(GTX100398) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
DNA polymerase gamma antibody detects DNA polymerase gamma protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA polymerase gamma antibody (GTX100398) diluted by 1:1000.
DNA polymerase gamma antibody detects POLG protein by western blot analysis.
A. 50 μg Rat testis lysate/extract
5% SDS-PAGE
DNA polymerase gamma antibody (GTX100398) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunofluorescence analysis of methanol-fixed HeLa, using DNA polymerase gamma(GTX100398) antibody at 1:200 dilution.
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文献和实验We report a simple homogeneous fluorescence assay for quantification of DNA polymerase function in high throughput. The fluorescence signal is generated by the DNA polymerase triggering opening of a molecular beacon extension of the template
The Polymerase Chain Reaction (PCR)
. Did He Really Invent PCR? • The basic principle of replicating a piece of DNA using two primers had already been described by Gobind Khorana in 1971:– Kleppe et al. (1971) J. Mol. Biol. 56, 341-346. • Progress was limited by primer synthesis and polymerase
能催化从四种5′-脱氧核苷三磷酸游离出焦磷酸而使DNA进行聚合反应的酶。EC2.7.7.7.。必须以DNA为模板，合成的DNA与模板具有互补的碱基排列（碱基互补性）。是与DNA复制和修复有关的重要的酶，虽可从许多组织分离出来，但对大肠杆菌的这种酶的研究较为先进。康伯格（A.Kornberg，1958）最初从大肠杆菌将此酶纯化，并弄清了其结构和特异性，但以后经过分子遗传学的研究，已明确了这种酶主要与DNA修复有关。因此重新进行与DNA复制有关酶的检索，发现两种新的酶。康伯格最初发现的酶称为
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