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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXA1. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Monopterus albus, Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX100308
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IP, ChIP assay, ChIP-seq
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
FOXA1
- 抗体英文名:
FOXA1 antibody
- 抗体名:
FOXA1 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FOXA1 antibody (GTX100308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
FOXA1 antibody detects FOXA1 protein at nucleus on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
FOXA1 antibody (GTX100308) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FOXA1 antibody (GTX100308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
FOXA1 antibody detects FOXA1 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
FOXA1 antibody (GTX100308) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
FOXA1 antibody immunoprecipitates FOXA1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 40 μg whole cell lysate/extract of FOXA1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FOXA1 by 2.5 μg of FOXA1 antibody (GTX100308) 10% SDS-PAGE The immunoprecipitated FOXA1 protein was detected by FOXA1 antibody (GTX100308) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-FOXA1 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.
Immunofluorescence analysis of PFA-fixed HeLa, using FOXA1(GTX100308) antibody at 1:1000 dilution.
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文献和实验Sundararaman B et al., Mol Cell 2016 (PMID:26990993)
Roza Berhanu Lemma et al., Epigenetics Chromatin 2022 (PMID:35440061)
Dylane Detilleux et al., Epigenetics Chromatin 2022 (PMID:35331302)
Xin Wang et al., Cell Biosci 2020 (PMID:33292595)
Lin ZS et al., Am J Cancer Res 2018 (PMID:30416851)
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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