Tartrate Resistant Acid Phosphatase / ACP5 蛋白|Tartrate Resistant Acid Phosphatase / ACP5 protein|Tartrate Resistant Acid Phosphatase / ACP5(Mouse, His Tag)
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- Sino Biological
- 50274-M08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
20 µg
蛋白名称：ACP5蛋白, ACP5 protein
蛋白构建：A DNA sequence encoding the pro form of mouse ACP5 (NP_031414.1) (Met 1-Pro 327) was fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP) . The specific activity is >9,000 pmoles/min/μg.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ala 23
蛋白分子量：The recombinant mouse ACP5 consists of 316 amino acids and has a predicted molecular mass of 35.8 kDa as estimated in SDS-PAGE under reducing conditions.
蛋白NP号：NP_031414.1
蛋白氨基酸序列：Met1-Pro327
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验colour. For demonstrating tartrate resistent acid phosphatase add 28mg/10ml of sodium tartrate to the incubating solution. Remember to use a control batch without sodium tartrate using test and control sections. NOTE
Bicinchoninic Acid (BCA) Protein Assay (Smith)
Considerations for use The bicinchoninic acid (BCA)assay is available in kit form from Pierce (Rockford,Ill.).This procedure is very applicable to microtiter plate methods.The BCA is used for the same reasons the Lowry is used.Stoscheck (1990
The Isolation of Osteoclasts from Human Giant Cell Tumors and Long-Term Marrow Cultures
of tartrate-resistant acid phosphatase, and response to calciotropic hormones do not distinguish effectively between osteoclasts and non-osteo-clastic cells The only unequivocal evidence of osteoclast activity is the ability of the cells to excavate authentic
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