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- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse ICAM-1 / CD54 Protein (His & Fc Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50.00 µg/100.00 µg
| 规格： | 50.00 µg | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100.00 µg | 产品价格： | ￥4520.0 |
蛋白名称：ICAM-1蛋白, ICAM-1 protein
蛋白构建：A DNA sequence encoding the extracellular domain of mouse ICAM1 (NP_034623.1) (Met 1-Asn 485) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 96 % as determined by SDS-PAGE
蛋白活性：Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to mouse ICAM1 coated plates (12.5 μg/ml, 100 μl/well), approximately >50% cells will adhere specifically .
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 28
蛋白分子量：The recombinant mouse ICAM1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 706 amino acids and has a predicted molecular mass of 78.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm ICAM1/Fc monomer is approximately 120-130 kDa due to glycosylation.
蛋白NP号：NP_034623.1
蛋白氨基酸序列：Met1-Asn485
蛋白标签：C-human IgG1-Fc & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Hu Y, et al. DNA Origami Tension Sensors (DOTS) to study T cell receptor mechanics at membrane junctions.bioRxiv : the preprint server for biology, PubMed ID: 37503090
3, Caillier A, et al. T cells Use Focal Adhesions to Pull Themselves Through Confined Environments.bioRxiv : the preprint server for biology, PubMed ID: 37904911
4, Hu Y, et al. Quantifying T cell receptor mechanics at membrane junctions using DNA origami tension sensors.Nature nanotechnology, PubMed ID: 39103452
CD11a/CD11b/CD11c 分子 CDlla 常用单克隆抗体或代号：MHM24， 2F12； CRIS―3(LFA―lα链，整合素α1) 主要表达细胞：Leu[A5] 分子质量(kDa)和结构：spl80(整合素α) 功 能：与1CAM-l(CD54)、ICAM-2(CDl02)、ICAM-3(C1350)结合，介导细胞黏附；与JAMl结合，参与白细胞穿过内皮细胞
IgE Fc段受体：FcαR 细胞因子受体 IL-1R（CDw121a）,IL-6R(CD126),M-CSFR(CD115),G-CSFR、SCFR(CD117)，PDGFR 白细胞分化抗原 CD2，LFA-3（CD58），ICAM-1（CD54），ICAM-2（CD102）、ICAM-3（CD50），CD4，CD8α、β链，CD28，B7/BB1（CD
,2F12;CRIS-3 Leu LFA-1(gp180/95)的α链 与ICAM-1（CD54）和ICAM-2（CD102）结合 CD11b Mol,OKM1; (Mac-1,CR3,integrin αm G,M,NK gp165/95的α链 C3bi和FB受体，与ICAM-1结合，粘附，调理吞噬 CD11c LeuM5;(CR4,integrin αx) M,G,NK,Tsub gp150/95的α链 C3bi受体，调理吞噬
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