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100 µg
蛋白名称:ESAM蛋白, ESAM protein
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Ala 248) of human ESAM (NP_620411.2) precursor was expressed,with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 97 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gln 30
蛋白分子量:The recombinant human ESAM consists of 230 amino acids and predicts a molecular mass of 25.4 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rh ESAM is approximately 35-40 kDa due to glycosylation.
蛋白NP号:NP_620411.2
蛋白氨基酸序列:Met1-Ala248
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Adhesion Molecule Expression on Cytokine-Stimulated Human Endothelial Cells
Leukocyte adhesion to vascular endothelial cells is central in regulating inflammatory responses. Endothelial cells line the vasculature and cover over 1000 m2 in the average adult (1 ). These cells were long thought to be inert, acting
Single-Molecule Methods to Study Cell Adhesion Molecules
forces of cell adhesion molecules, which may shed insights on molecular mechanisms of cellular adhesion. In addition, single-molecule techniques enable us to observe the detailed distributions of individual molecular behaviors that cannot be readily
细胞粘着分子(cell adhesion molecule, CAM)
参与细胞粘着的分子称为细胞粘着分子。 自从N-CAM被发现之后,陆续发现了一些新的细胞粘着分子,如Ng-CAM是神经胶质细胞粘着分子,L-CAM是肝细胞的细胞粘着分子,I-CAM是普遍存在的一种细胞粘着分子,它的配体是LFA-1;而LEC-CAM是白细胞及其它循环细胞中发现的细胞粘着分子。上述细胞粘着分子都是糖蛋白,在细胞外结构域都有与肽共价结合的糖基。根据细胞粘着分子的作用方式可分为三个家族:①免疫球蛋白超家族,如V-CAM、Ng-CAM、I-CAM和L1等;②钙粘着蛋白家族,如E
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