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100 µg
蛋白名称:interferon-alpha/IFNA5蛋白, interferon-alpha/IFNA5 protein
蛋白构建:A DNA sequence encoding the human IFNα G (NP_002160.1) (Leu 22-Glu 189) was fused with the Fc region of human IgG1 at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV). The EC50 for this effect is 0.16-0.8 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 20
蛋白分子量:The recombinant human IFNα G/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 405 amino acids with a predicted molecular mass of 46.3 kDa. As a result of glycosylation, rh IFNα G/Fc monomer migrates as an approximately 48-50 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_002160.1
蛋白氨基酸序列:Leu22-Glu189
蛋白标签:N-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验motifs located prevalently in the 3′ untranslated regions of target messenger RNAs (mRNA). Interferon-alpha-2a (IFNα) induces a large set of protein coding genes mediating antiproliferative and antiviral responses. Here we use a global microarray-based
Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
the isolation of activated Fc receptor complexes from RAW 264.7 macrophages using live?cell affinity receptor chromatography (LARC). The Fc receptor complex is activated and captured by IgG?coated microbeads on the surface of live macrophages. After the cells
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