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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human Latent TGF-beta 1 / TGFB1 Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/10.00 µg/20.00 µg/50.00 µg/500.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥32200.0 |
|---|---|---|---|
| 规格: | 10.00 µg | 产品价格: | ¥1400.0 |
| 规格: | 20.00 µg | 产品价格: | ¥2310.0 |
| 规格: | 50.00 µg | 产品价格: | ¥2980.0 |
| 规格: | 500.00 µg | 产品价格: | ¥19380.0 |
重组人 Latent TGF-beta 1 / TGFB1 蛋白 (His标签)(产品说明)
蛋白名称:Human Latent TGF-beta 1 / TGFB1 Protein (His Tag)
蛋白构建:A DNA sequence encoding the full length of human TGFβ1 (NP_000651.3) (Met 1-Ser 390) was fused with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized Human TGF beta 1 His (Cat: 10804-H08H) at 2 μg/ml (100 μl/well) can bind Human TGFBR2 His & hFc (Cat: 10358-H03H), the EC50 of Human TGFBR2 His & hFc is 5-35 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Leu 30 & Ala 279
蛋白分子量:The recombinant human latent TGFβ1 consists of 370 amino acids and has a calculated molecular mass of 42.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TGFβ1 is approximately 16, 38 and 55 kDa corresponding to mature TGFβ1, LAP protein and inacitve latent TGFβ1 respectively due to glycosylation. In non-reduced SDS-PAGE, it migrates as an approximately 110 kDa protein consisting of a TGFβ1 homodimer non-covalently linked to a LAP homodimer.
蛋白NP号:NP_000651.3
蛋白氨基酸序列:Met1-Ser390
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称:Human Latent TGF-beta 1 / TGFB1 Protein (His Tag)
蛋白构建:A DNA sequence encoding the full length of human TGFβ1 (NP_000651.3) (Met 1-Ser 390) was fused with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized Human TGF beta 1 His (Cat: 10804-H08H) at 2 μg/ml (100 μl/well) can bind Human TGFBR2 His & hFc (Cat: 10358-H03H), the EC50 of Human TGFBR2 His & hFc is 5-35 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Leu 30 & Ala 279
蛋白分子量:The recombinant human latent TGFβ1 consists of 370 amino acids and has a calculated molecular mass of 42.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TGFβ1 is approximately 16, 38 and 55 kDa corresponding to mature TGFβ1, LAP protein and inacitve latent TGFβ1 respectively due to glycosylation. In non-reduced SDS-PAGE, it migrates as an approximately 110 kDa protein consisting of a TGFβ1 homodimer non-covalently linked to a LAP homodimer.
蛋白NP号:NP_000651.3
蛋白氨基酸序列:Met1-Ser390
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验该产品被引用文献
1, Yang, P; et al. Comprehensive Analysis of Fibroblast Activation Protein (FAP) Expression in Interstitial Lung Diseases (ILDs). American journal of respiratory and critical care medicine, PMID: 35984444
2, Zhang, X; et al. Fu-Zheng-Tong-Luo formula promotes autophagy and alleviates idiopathic pulmonary fibrosis by controlling the Janus kinase 2/signal transducer and activator of transcription 3 pathway. Journal of ethnopharmacology, PMID: 37207878
3, Shen, G; et al. Kallistatin Deficiency Induces the Oxidative Stress-Related Epithelial-Mesenchymal Transition of Retinal Pigment Epithelial Cells: A Novel Protagonist in Age-Related Macular Degeneration. Investigative ophthalmology & visual science, PMID: 37682567
4, Green, A; et al. Dynamic three dimensional environment for efficient and large scale generation of smooth muscle cells from hiPSCs. Stem cell research & therapy, PMID: 39627821
5, Wei, G; et al. Cancer-associated fibroblasts induce almonertinib resistance in non-small cell lung cancer. Journal of translational medicine, PMID: 39794783
6, Zhang, M; et al. circSIRT2/miR-542-3p/VASH1 axis regulates endothelial-to-mesenchymal transition (EndMT) in subretinal fibrosis in age-related macular degeneration models. Aging cell, PMID: 39744871
7, Zhou, J; et al. Fibroblast growth factor 18 attenuates renal fibrosis via AMPK/NOX4 pathway in mice. Biochemical and biophysical research communications, PMID: 40311293
2, Zhang, X; et al. Fu-Zheng-Tong-Luo formula promotes autophagy and alleviates idiopathic pulmonary fibrosis by controlling the Janus kinase 2/signal transducer and activator of transcription 3 pathway. Journal of ethnopharmacology, PMID: 37207878
3, Shen, G; et al. Kallistatin Deficiency Induces the Oxidative Stress-Related Epithelial-Mesenchymal Transition of Retinal Pigment Epithelial Cells: A Novel Protagonist in Age-Related Macular Degeneration. Investigative ophthalmology & visual science, PMID: 37682567
4, Green, A; et al. Dynamic three dimensional environment for efficient and large scale generation of smooth muscle cells from hiPSCs. Stem cell research & therapy, PMID: 39627821
5, Wei, G; et al. Cancer-associated fibroblasts induce almonertinib resistance in non-small cell lung cancer. Journal of translational medicine, PMID: 39794783
6, Zhang, M; et al. circSIRT2/miR-542-3p/VASH1 axis regulates endothelial-to-mesenchymal transition (EndMT) in subretinal fibrosis in age-related macular degeneration models. Aging cell, PMID: 39744871
7, Zhou, J; et al. Fibroblast growth factor 18 attenuates renal fibrosis via AMPK/NOX4 pathway in mice. Biochemical and biophysical research communications, PMID: 40311293
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重组人 Latent TGF-beta 1 / TGFB1蛋白 (His 标签)
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