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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human CD38 Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
10.00 µg/20.00 µg/50.00 µg/500.00 µg
| 规格: | 10.00 µg | 产品价格: | ¥1790.0 |
|---|---|---|---|
| 规格: | 20.00 µg | 产品价格: | ¥3220.0 |
| 规格: | 50.00 µg | 产品价格: | ¥3150.0 |
| 规格: | 500.00 µg | 产品价格: | ¥11390.0 |
重组人 CD38 蛋白 (His标签)(产品说明)
蛋白名称:Human CD38 Protein (His Tag)
蛋白构建:A DNA sequence encoding the extracellular domain of human CD38 (NP_001766.2) (Val 43-Ile 300) with a C-terminal polyhistidine tag was expressed.
表达宿主:HEK293 Cells
蛋白纯度:> 97 % as determined by SDS-PAGE
蛋白活性:1. Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDPribose.The specific activity is > 2,500 pmoles/min/μg. 2. Measured by its binding ability in a functional ELISA. Immobilized Human CD38 His (Cat:10818-H08H) at 2 μg/ml (100 μl/well) can bind Mouse Anti-CD38 Antibody (Cat:10818-MM03), the EC50 of Mouse Anti-CD38 Antibody is 30-120 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Val 43
蛋白分子量:The secreted recombinant human CD38 comprises 269 amino acids with a predicted molecular mass of 31.3 kDa. As a result of glycosylation, rhCD38 migrates as an approximately 43-45 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_001766.2
蛋白氨基酸序列:Val43-Ile300
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Liu, L; et al. A novel approach to remove interference of therapeutic monoclonal antibody with serum protein electrophoresis. Clinical biochemistry, PMID: 31669513
3, Luo, YR; et al. A Thin-Film Interferometry-Based Label-Free Immunoassay for the Detection of Daratumumab Interference in Serum Protein Electrophoresis. Clinica chimica acta; international journal of clinical chemistry, PMID: 31883925
4, Ugamraj, HS; et al. TNB-73
8, a biparatopic antibody, boosts intracellular NAD+ by inhibiting CD38 ecto-enzyme activity. mAbs, PMID: 35867844
5, Li, Y; et al. Discovery of a First-in-Class CD38 Inhibitor for the Treatment of Mitochondrial Myopathy. Journal of medicinal chemistry, PMID: 37696000
植物源重组人胰岛素生长因子融合蛋白(OsrIGF-1),胰岛素在无血清细胞培养中的最佳替代品。 IGFs家族由两种低分子多肽(IGF-Ⅰ、IGF-Ⅱ)、两类特异性受体及六种结合蛋白组成。IGF-Ⅰ是一个有70个氨基酸的单链碱性蛋白,分子量7649Da,耐热。IGFs的生物学功能不只局限于有丝分裂刺激作用,它们也能诱导分化或促进分化功能的表达。全新推出的植物源重组人类胰岛素生长因子-1融合蛋白(OsrIGF-1)在IGF-1的N末端融合了人血清白蛋白(HSA),HSA作为蛋白质保护剂增加了IGF
His 标签蛋白纯化效果不理想,宝宝心里苦呀。今天,小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面: 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高,容易导致蛋白炭化;功率过低,蛋白释放不出来。 建议 改变超声功率,同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素,如:金属离子螯合剂、强还原剂、过高的咪唑浓度。同时,优化缓冲液的pH、盐
Purification of 6xHis epitope tagged proteins by Ni-NTA-Agarose His标签蛋白纯化
Low (“Low”)Imidazole Buffer 0.5L 100mM Imidizole 3.4g 5% glycerol 25ml 100% glycerol 50mM Tris-HCl (pH 7.9)50ml 0.5M Tris-HCl (pH 7.9) 0.1% Tween-20 0.5M 100% Tween-20 500mM NaCl50ml 5M NaCl dH2O Fill to 0.5L (start w/ 350ml) High (“High
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