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100 µg
蛋白名称:ICOS ligand蛋白, ICOS ligand protein
蛋白构建:A DNA sequence encoding the mouse B7-H2 (NP_056605.1) extracellular domain (Met 1-Lys 279) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA . Immobilized human ICOS at 1 μg/ml (100 μl/well) can bind biotinylated mouse B7-H2 Fc chimera with a linear range of 0.125-1.0 μg/ml .
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 47
蛋白分子量:The secreted recombinant mouse B7-H2/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer comprises 481 amino acids with a predicted molecular mass of 54.3 kDa. As a result of glycosylation, it migrates as an approximately 75-85 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_056605.1
蛋白氨基酸序列:Met1-Lys279
蛋白标签:C-human IgG1-Fc & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验同锚定蛋白结合的任何分子都称为配体。在受体介导的内吞中, 与细胞质膜受体蛋白结合, 最后被吞入细胞的即是配体。根据配体的性质以及被细胞内吞后的作用, 将配体分为四大类:Ⅰ.营养物, 如转铁蛋白、低密度脂蛋白(LDL)等; Ⅱ.有害物质, 如某些细菌; Ⅲ.免疫物质, 如免疫球蛋白、抗原等; Ⅳ.信号物质, 如胰岛素等多种肽类激素等。
Ligand- and Structure-Based Pregnane X Receptor Models
The human pregnane X receptor (PXR) is a ligand dependent transcription factor that can be activated by structurally diverse agonists including steroid hormones, bile acids, herbal drugs, and prescription medications. PXR regulates
describes a dye ligand chromatography technique for purifying proteins from overexpression, in mammalian cell culture. The method begins with batch binding in order to rapidly select binding and elution conditions. Subsequently, gradient elution is employed
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