- ￥580 - 3980
- Sino Biological
- 北京
- 10273-H01H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human PDGF-C Protein (Fc Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
5.00 µg/20.00 µg/100.00 µg
| 规格： | 5.00 µg | 产品价格： | ￥580.0 |
|---|---|---|---|
| 规格： | 20.00 µg | 产品价格： | ￥1380.0 |
| 规格： | 100.00 µg | 产品价格： | ￥3980.0 |
蛋白名称：PDGF-C蛋白, PDGF-C protein
蛋白构建：A DNA sequence encoding the mature form of human PDGF-C (NP_057289.1) (Val 235-Gly 345) was expressed with the fused Fc region of human IgG1 at the N-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.5-3 μg/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Glu 20
蛋白分子量：The recombinant human PDGFC/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 348 amino acids and predicts a molecular mass of 39 kDa. As a result of glycosylation, rh PDGFC/Fc monomer is approximately 45 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：NP_057289.1
蛋白氨基酸序列：Val235-Gly345
蛋白标签：N-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Wang J, et al. Intervention of exogenous VEGF protect brain microvascular endothelial cells from hypoxia-induced injury by regulating PLCγ/RAS/ERK and PI3K/AKT pathways.Experimental gerontology, PubMed ID: 38718888
. This purification process resulted in greater than 95% pure rBoNTC(Hc )-C based on SDS-PAGE, and yielded up to 0.2 g of rBoNTC(Hc )-C/kg cells. The final protein yield is a function of protein expression level during fermentation and the purification methods
Strep-tag®技术是亲和纯化重组蛋白的常用工具，它基于自然界中最强的非共价相互作用之一，即生物素与链霉亲和素的相互作用。该纯化系统的突出特点是纯化过程温和、目的蛋白纯度高、特异性强、兼容多种表达系统，对于有挑战性蛋白的纯化十分有帮助，因此近些年来脱颖而出。 本文将从以下方面介绍Strep-tag®技术： 标签介绍 纯化原理 标签-配体 纯化步骤 系统特点 标签介绍 目前有2种广泛使用的Strep标签： 它们分别是： Strep-tag®II 8 aa小标签(Trp-Ser
Expression of Recombinant Cytochromes c in E. coli
for proper folding and stability. However, significant advances in expression of recombinant cytochromes c have been made during the last decade. It has been shown that a single gene cluster, ccmA–H , is responsible for cytochrome c maturation in Escherichia
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