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Rat IL-10 ELISA Kit (大鼠白细胞介素10

ELISA试剂盒)
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  • ¥1600 - 2650
  • MultiSciences已认证
  • 62.5 pg/mL - 4000 pg/mL
  • 浙江杭州
  • EK310
  • 2025年12月23日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      99

    • 供应商

      联科生物

    • 检测范围

      62.5 pg/mL - 4000 pg/mL

    • 检测方法

      双抗夹心法

    • 应用

      ELISA

    • 适应物种

      Rat

    • 标记物

      HRP

    • 样本

      血清,血浆,细胞培养上清及其他生物学样本

    • 规格

      96T/48T

    规格:96T产品价格:¥2650.0
    规格:48T产品价格:¥1600.0

    Rat IL-10 ELISA Kit

    描述

    商品名

    Rat IL-10 ELISA Kit (大鼠白细胞介素10 ELISA试剂盒)

    检测方法

    双抗夹心法

    精密度

    板内变异系数:3.4% - 5.3%;板间变异系数:4.5% - 6.1%

    样本类型

    血清,血浆,细胞培养上清及其他生物学样本

    检测样本体积

    20 μL

    灵敏度

    0.48 pg/mL

    检测范围

    62.5 pg/mL - 4000 pg/mL

    回收率

    86% - 108%

    平均回收率

    0.98

    板式

    96孔板,可拆

    保存

    试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

    运输条件

    4℃蓝冰运输

    组分
    • 包被抗IL-10单克隆抗体的96孔聚苯乙烯酶标板
    • 大鼠IL-10冻干标准品
    • IL-10检测抗体
    • 标准品稀释液
    • 检测缓冲液(10×)
    • 底物(TMB)
    • 终止液
    • 洗液(20×)
    • 封板膜

    检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-10与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-10的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长5630 nm)测定吸光度值。

    分子信息

    概述大鼠 IL10, CSIF, TGIF 靶点信息

    IL10 分子靶点信息概述

    • 分子名:IL10, interleukin 10
    • 基因家族:Interleukins
    • 别名:CSIF; TGIF; IL10A; IL-10
    • 全称:cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

    IL10 分子靶点综述

    白细胞介素10(IL-10)是由2个长178个氨基酸的亚基构成的同源二聚体。在人类中,它由位于1号染色体的IL-10基因编码,由5个外显子组成。IL-10主要由单核细胞分泌,淋巴细胞、II型辅助性T细胞(Th2)、肥大细胞、CD4+CD25+Foxp3+调节性T细胞和部分活化的T细胞和B细胞分泌较少。它对免疫调控和炎症具有多种效应。它下调巨噬细胞的Th1细胞因子、II类MHC抗原和共刺激分子。它还能增强B细胞存活、增殖和抗体分泌。IL-10能阻断NF-κB活性,并参与JAK-STAT信号通路调控。IL-10的免疫抑制特性提示其可能应用于临床,抑制器官移植后的排斥。IL-10还具有强烈的消炎特性。

    大鼠 Rat Il10 分子靶点信息

    • 分子名:Il10, interleukin 10
    • 别称:
      • CSIF
      • cytokine synthesis inhibitory factor
      • If2a
      • IL-10
      • IL10X
      • interferon 2a
      • interleukin-10
    • 基因序列:NCBI_Gene: 25325
    • 蛋白序列:
      • UniprotKB: A0A7R8C3K4
      • UniprotKB: P29456

    大鼠 Rat Il10 靶点分子功能(预测)

    Enables interleukin-10 receptor binding activity. Involved in several processes, including cellular response to estradiol stimulus; liver regeneration; and negative regulation of nitric oxide biosynthetic process. Located in extracellular space. Used to study several diseases, including lung disease (multiple); perinatal necrotizing enterocolitis; sciatic neuropathy; toxic encephalopathy; and transient cerebral ischemia. Biomarker of several diseases, including artery disease (multiple); decubitus ulcer; lung disease (multiple); obstructive sleep apnea; and postpartum depression. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

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    Rat IL-10 ELISA Kit (大鼠白细胞介素10 ELISA试剂盒)
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