- ￥2000 - 3400
- MultiSciences
- 3.13 pg/mL - 200 pg/mL
- 70-EK304HS
- 浙江杭州
- 2025年12月19日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
联科生物
- 库存：
99
- 样本：
血清，血浆，细胞培养上清及其他生物学样本
- 标记物：
HRP
- 适应物种：
Rat
- 应用：
ELISA
- 检测方法：
双抗夹心法
- 检测范围：
3.13 pg/mL - 200 pg/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥2000.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥3400.0 |
描述
| 商品名 | Rat IL-4 High Sensitivity ELISA Kit (大鼠白细胞介素4高敏 ELISA试剂盒) |
|---|---|
| 检测方法 | 双抗夹心法 |
| 精密度 | 板内变异系数：5.0% - 6.8%；板间变异系数：2.7% - 7.5% |
| 样本类型 | 血清，血浆，细胞培养上清及其他生物学样本 |
| 检测样本体积 | 20 μL |
| 灵敏度 | 0.20 pg/mL |
| 检测范围 | 3.13 pg/mL - 200 pg/mL |
| 回收率 | 81% - 101% |
| 平均回收率 | 0.9 |
| 板式 | 96孔板，可拆 |
| 保存 | 试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 | 4℃蓝冰运输 |
| 组分 |
|
检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-4抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-4与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-4的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
标准曲线
分子信息
概述大鼠 IL4, IL-4, BSF1, BCGF1 靶点信息IL4 分子靶点信息概述
- 分子名：IL4, interleukin 4
- 基因家族：Interleukins
- 别名：BSF1; IL-4; BCGF1; BCGF-1; MGC79402
- 全称：B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; B cell growth factor 1
IL4 分子靶点综述
IL-4是II型辅助T细胞(Th2细胞)分泌的细胞因子。IL-4是一种多效性细胞因子，在调节T、B淋巴细胞和其他类型细胞的增殖、分化、凋亡，促进以Th2细胞为特征的免疫应答过程中发挥重要作用。IL-4多重生物效应依赖于细胞类型和分化状态，研究表明其在介导过敏性疾病、自身免疫性疾病、感染性疾病、肿瘤等疾病的免疫反应中有多重作用，对肿瘤、自身免疫性疾病和感染性疾病等有治疗作用，并且IL-4对疫苗免疫应答具有调节作用。
大鼠 Rat Il4 分子靶点信息
- 分子名：Il4, interleukin 4
- 别称：
- B-cell growth factor 1
- B-cell IGG differentiation factor
- B-cell stimulatory factor 1
- BSF-1
- IL-4
- Il4e12
- interleukin-4
- lymphocyte stimulatory factor 1
- 基因序列：NCBI_Gene: 287287
- 蛋白序列：
- UniprotKB: A0A0G2JVY1
- UniprotKB: P20096
大鼠 Rat Il4 靶点分子功能（预测）
Predicted to enable cytokine activity. Involved in several processes, including positive regulation of biosynthetic process; positive regulation of leukocyte migration; and regulation of defense response. Located in extracellular space. Used to study anti-basement membrane glomerulonephritis; autoimmune thyroiditis; and pelvic inflammatory disease. Biomarker of several diseases, including cerebrovascular disease (multiple); interstitial lung disease (multiple); irritant dermatitis; primary biliary cholangitis; and rhinitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).
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- 作者
- 内容
- 询问日期
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- IF = 7.561 Probiotic Interventions Alleviate Food Allergy Symptoms Correlated With Cesarean Section: A Murine Model. Frontiers in Immunology (2021)
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- IF = 4.412 The Effect of Protein-Rich Extract from Bombyx Batryticatus against Glutamate-Damaged PC12 Cells Via Regulating γ-Aminobutyric Acid Signaling Pathway. MOLECULES (2020)
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- IF = 4.162 Orally Deliverable Dual-Targeted Pellets for the Synergistic Treatment of Ulcerative Colitis. Drug Design Development and Therapy (2021)
- IF = 3.943 DiR-labeled tolerogenic dendritic cells for targeted imaging in collagen- induced arthritis rats. INTERNATIONAL IMMUNOPHARMACOLOGY (2020)
- IF = 3.861 Moxibustion regulates the polarization of macrophages through the IL-4/STAT6 pathway in rheumatoid arthritis. CYTOKINE (2022)
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RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
on the original ELISA principle, this chapter will focus on the two perhaps most useful and routinely performed: the indirect sandwich ELISA, providing high sensitivity and specificity; and the basic direct ELISA, useful when only one antibody to the sample
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