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- Sino Biological
- 11216-HNCE
- 2025年08月12日
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- 文献和实验
- 技术资料
- 规格：
100 µg
蛋白名称：RIZ1蛋白, RIZ1 protein
蛋白构建：A DNA sequence encoding the N-terminal segment of human PRDM2 (NP_036363.2) (Met 1-Ala 200), containing the SET domian, was expressed, with two additonal amino acids (Gly & Pro) at the N-terminus.
表达宿主：E. coli
蛋白纯度：> 96 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：Please contact us for more information.
预测N端：Gly
蛋白分子量：The recombinant human PRDM2 consists of 202 amino acids and predicts a molecular mass of 23 kDa. It migrates as an approximately 26 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_036363.2
蛋白氨基酸序列：Met1-Ala200
蛋白标签：N-cleavage
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验James Hardwick CNBr Cleavage Procedure
1. Immunoprecipitate the protein and run it on a preparative gel. CNBr cleavage must be done with protein transferred to a nitrocellulose filter. Neither Immobilon nor Nylon can substitute. IMPORTANT: Wash the NC 2X for 15 min
Thrombin Cleavage of GST-Fusion protein
INTRODUCTION In many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The amount of thrombin, temperature and length of incubation must be calibrated
Factor Xa Cleavage of MBP-Fusion protein
INTRODUCTION In many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The amount of factor Xa, temperature and length of incubation must be calibrated
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