- ￥2310 - 3870
- Sino Biological
- 北京
- 10339-H08E
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human SMAC / Diablo Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100.00 µg/200.00 µg
| 规格： | 100.00 µg | 产品价格： | ￥2310.0 |
|---|---|---|---|
| 规格： | 200.00 µg | 产品价格： | ￥3870.0 |
蛋白名称：SMAC蛋白, SMAC protein
蛋白构建：A DNA sequence encoding the mitochondrial-located form of human Diablo (NP_063940.1) (Ala 56-Asp 239) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：E. coli
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized recombinant human SMAC-His (Cat:10339-H08E）at 10 μg/ml (100 μl/well) can bind recombinant human XIAP-AVI (Cat:10606-H17E) with a linear range of 0.125-1.0 μg/ml.
蛋白内毒素：Please contact us for more information.
预测N端：Met
蛋白分子量：The recombinant human Diablo/SMAC consists of 191 amino acids and migrates as polypeptide of 22 kDa as estimated by SDS-PAGE under reduced and non-reduced conditions.
蛋白NP号：NP_063940.1
蛋白氨基酸序列：Ala56-Asp239
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Chromatin Interaction Analysis Using Paired‐End Tag Sequencing
Analysis using Paired?End Tag sequencing (ChIA?PET) is a technique developed for large?scale, de novo analysis of higher?order chromatin structures. Cells are treated with formaldehyde to cross?link chromatin interactions, DNA segments bound by protein
.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
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