Recombinant Human VEGFR2 / Flk-1 / CD309 / KDR Protein (Fc Tag)
库存:
99
供应商:
北京义翘神州科技股份有限公司
规格:
100 µg/1 mg
规格:
100 µg
产品价格:
¥4520.0
规格:
1 mg
产品价格:
¥29480.0
重组人 VEGFR2 / Flk-1 / CD309 / KDR 蛋白 (Fc标签)(产品说明)
蛋白名称:Human VEGFR2 / Flk-1 / CD309 / KDR Protein (Fc Tag)
蛋白构建:A DNA sequence encoding the extracellular domain of human VEGFR2 (NP_002244.1) (Met 1-Glu 764) was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:1. Immobilized Human VEGF121 (Cat:10008-HNAH) at 2 μg/mL (100 μL/well) can bind Human VEGFR2/KDR (ECD, hFc Tag)(Cat:10012-H02H), the EC50 of Human VEGFR2/KDR (ECD, hFc Tag)(Cat:10012-H02H) is 6-48 ng/mL.2. Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 20-120 ng/mL in the presence of 10 ng/mL recombinant human VEGF165.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ala 20
蛋白分子量:The recombinant human VEGFR2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 979 amino acids and predicts a molecular mass of 109 kDa. The apparent molecular mass of human VEGFR2/Fc monomer is approximatly 150-160 KDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:NP_002244.1
蛋白氨基酸序列:Met1-Glu764
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
1, Li, JJ; et al. Development and Characterization of the Recombinant Human VEGF-EGF Dual-Targeting Fusion Protein as a Drug Delivery System. Bioconjug. Chem., Pubmed: 26525951