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100 µg
蛋白名称:Growth Hormone Receptor蛋白, Growth Hormone Receptor protein
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Gln 273) of mouse GHR (NP_034414.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to inhibit proliferation of INS-1 cells induced by human growth hormone. The ED50 for this effect is 0.5-2 μg/mL in the presence of 50 ng/mL human growth hormone.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Thr 25
蛋白分子量:The recombinant mouse GHR/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 497 amino acids and has a predicted molecular mass of 56.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmGHR/Fc monomer is approximately 70-80 kDa due to glycosylation.
蛋白NP号:NP_034414.2
蛋白氨基酸序列:Met1-Gln273
蛋白标签:C-human IgG1-Fc & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验总蛋白(包括质膜和细胞器膜蛋白),但费时费力。目前,有一种最新型有效的膜蛋白提取工具,并具有高纯度、高特异性、高载量温和提取的优点。是基于1980年科学家在牛眼视网膜中发现的一种命名为Rho1D4的氨基酸序列以及其对应的抗体来作为亲和标签纯化膜蛋白取得了非常好的效果,将Rho1D4抗体链接在琼脂糖或磁珠上,用于亲和层析带有Rho1D4标签的膜蛋白,能近乎完美的解决这些研究难题。1) 什么是Rho1D4?Rho1D4是指在牛眼视网膜细胞内的牛视紫红质C端的最后九位氨基酸。Rho1D4得名于与该序列
receptor type I is designed and validated. This experimental procedure was used to determine the abundance of growth hormone receptor type I transcript in different tilapia tissues. The results obtained with this developed competitive RT-PCR were similar
笔者前一段时间忙于为新文章制作配图,多处搜集配图优雅美观的优质论文。最后终于找到一篇发表在 Science Advances 的文章——通过高通量测序分析揭示了小 RNA 在小鼠卵母细胞和早期胚胎中的调控作用。文章中除了复杂的 RNA Seq、Chip Seq 分析,图表的搭配十分美观。虽说我们是崇高的科(ke)学 (yan) 家 (gou),但关乎我们毕业大计的 SCI 也得要「高颜值」。以常见的热图(heatmap)为例,在分子生物学涉及高通量以及芯片的文章中,尤其是 RNA-seq 相关
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