重组大鼠 ALK-1 / ACVRL1 蛋白 (His & Fc标签)(产品说明)
蛋白名称:Rat ALK-1 / ACVRL1 Protein (His & Fc Tag)
蛋白构建:A synthetic DNA sequence encoding the rat ACVRL1 (NP_071886.1) extracellular domain (Met 1-Ala 118) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953.The ED50 for this effect is typically 40-200 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Asp 17
蛋白分子量:The secreted recombinant rat ACVRL1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer comprises 343 amino acids with a predicted molecular mass of 38.7 kDa. As a result of glycosylation, it migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_071886.1
蛋白氨基酸序列:Met1-Ala118
蛋白标签:C-human IgG1-Fc & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.