Influenza A H3N2 (A/South Australia/34/2019) Hemagglutinin / HA Protein (ECD, His Tag)
库存:
大量
供应商:
北京义翘神州科技股份有限公司
规格:
100µg
Influenza A H3N2 (A/South Australia/34/2019) Hemagglutinin / HA Protein (ECD, His Tag): 产品信息
纯度:≥ 95 % as determined by SDS-PAGE.
内毒素:< 1.0 EU per μg protein as determined by the LAL method.
生物活性:Testing in progress
蛋白构建:A DNA sequence encoding the Influenza A virus ((A/South Australia/34/2019) (H3N2)) hemagglutinin (translated amino acids of EPI1607117) (Met1-Asp529), termed as HA, was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.
表达宿主:Baculovirus-Insect Cells
种属:H3N2
预测 N 端:Gln 17
分子量:The recombinant Influenza A virus ((A/South Australia/34/2019) (H3N2))Hemagglutinin / HA comprises 561 amino acids and has a predicted molecular mass of 63.22 kDa. It migrates as an approximately 80.93 kDa band in SDS-PAGE under reducing conditions.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶:A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Hemagglutinin/HA 背景信息 The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018