c-MET/HGFR重组蛋白|Recombinant Human c-MET / HGFR Protein (ECD, His & Fc Tag)

人 c-MET蛋白|c-MET protein|c-MET(Human, C-human IgG1-Fc & His)（产品说明）
蛋白名称：c-MET蛋白, c-MET protein
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Thr 932) of human c-Met (NP_000236) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGF at 1 μg/ml (100ul/well) can bind Human c-MET / HGFR with a linear range of 0.31-160ng/ml
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Glu 25
蛋白分子量：The mature recombinant human c-Met/Fc is a disulfide-linked tetramer composed of two proteolytically cleaved α and β subunits. Each α and β together with the C-terminal Fc tag consists of 1155 amino acids and has a predicted molecular mass of 129.5 (α =32.5 + Fc tagged β=97) kDa. The rh c-MET/Fc heterodimer thus migrates with apparent molecular mass of approximately 45 kDa and 120 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：NP_000236
蛋白氨基酸序列：Met1-Thr932
蛋白标签：C-human IgG1-Fc & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.