重组人 ACVR2B / ActivinR-IIB蛋白 (ECD, Fc 标签)

重组人 ACVR2B / ActivinR-IIB 蛋白 (ECD, Fc标签)（产品说明）
蛋白名称：Human ACVR2B / ActivinR-IIB Protein (ECD, Fc Tag)
蛋白构建：A DNA sequence encoding the N-terminal segment (Met 1-Thr 134) from the extracellular domain of human ACVR2B (NP_001097.2) was expressed with the fused human IgG1 Fc region at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：1. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.02-0.1 µg/mL in the presence of 10 ng/mL recombinant Activin A. 2. Measured by its binding ability in a functional ELISA. Immobilized Inhibin Human, Mouse, Rat, Cynomolgus, Rhesus Inhibin beta A/Activin A (Cat:10429-HNAH) at 2 μg/ml (100 μl/well) can bind Human ACVR2B hFc(Cat:10229-H02H), the EC50 of Human ACVR2B hFc is 12-60 ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ser 19
蛋白分子量：The recombinant human ACVR2B/Fc chimera is a disulfide-linked homodimer generated after removal of the signal peptide. The reduced monomer comprises 354 amino acids and predicts a molecular mass of 40.0 kDa. As a result of glycosylation, the monomer migrates as an approximately 60-65 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001097.2
蛋白氨基酸序列：Met1-Thr134
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.