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电子触觉测试仪也叫做电子测痛仪,用于对大鼠、小鼠足部进行轻触,来测量老鼠的机械痛阈值或触觉阈值。
老鼠感觉到爪子被刺激时,会做出缩足反应。电子触觉测试仪使用测试探针对老鼠足底进行触觉刺激,力度由小到大逐渐增加力度,通过判断老鼠的缩足反应,来采集和分析老鼠的触觉阈值。
用于测试触觉灵敏度的经典仪器是Semmes-Weinstein纤毛机械刺激丝套装。但是Semmes-Weinstein纤毛机械刺激丝的方法非常耗时耗力,一个测试实验需要使用多个不同纤维丝进行多次和反复足底刺激。
电子触觉测试仪可用来替代传统的Semmes-Weinstein(Von Frey Hairs)纤毛机械刺激针,可直接测得动物的机械痛阈和触觉阈测量,不需要进行反复的测试和繁杂的计算。设备轻巧易用,测量精确。
电子触觉测试仪相比传统方法的优势特点:
· 使用电子触觉测试仪进行一个测试实验,只需要进行一次足底刺激即可得到准确的结果;
· 数据可直接用于统计分析,能够节省很多的精力和时间;
· 测量精度高,测试力度能够读取到1000g以内的具体数值,精度可达0.1g;
· 不需要进行繁杂的统计和计算;
· 使用一个标准直径的刚性探针进行测试,能够避免不同力度纤维丝具有不同的直径而产生的触觉误差;
· 能够避免因为反复多次的测试对动物耐受力的影响,减小测试误差;
型号:38450
产品特点:
· 测试探针被固定在测试手柄上,通过测试探针自下而上对老鼠足底的刺激,老鼠会在刺激达到最大耐受力度时抬脚躲避,此时可通过脚踏开关来判断测试结果;
· 测试主机会显示和记录测试结果,包括最大测试力度和动物反应时间;
· 实验人员可根据本次测试老鼠的活动情况,接受或者拒绝本次测试结果;
· 被接受的测试结果会记录在主机中,可传到电脑进行统计;
· 为了方便测试,测试手柄端部采用了棱镜式设计,方便进行定位和瞄准;
· 设备中包含有助于稳定探针加力速率的软件工具;
主要参数:
· 最大适用力度:1000g
· 分辨率:0.1g
· 自动记录动物反应
· 辅助用户控制探针的加力速率
· 采用棱镜设计,有助于定位目标
电子触觉测试仪中使用的金属探针与经典的动态足底测试仪 Aesthe-siometer 37450 中使用的金属探针相同,可以对两种仪器的结果进行一致性比较。
带棱镜式设计的手柄,在进行“接触式刺激”
大鼠和小鼠测试探针细节
小鼠测试探针细节
测试手柄的主要特色:
具备有效的峰值检测器,自动捕捉和记录测试过程中施加的最大力度值;
具备可靠的自动检测动物反应功能;
具备辅助稳定探针加力速率的功能;
数据监测和存储:
测试主机具有存储和查看功能;
可将数据导出到电脑进行数据统计和分析;
可导出Excel或文本格式;
软件中可查看施加的力(红线)、所需的目标力率(蓝线)和峰值检测
基本配置:
带棱镜和刺激传感器的测试手柄,测试主机,脚踏板、软件和USB电缆。
选配:
测试支架



大鼠、小鼠测试笼
参考文献:
【1】Pain Relieving and Neuroprotective Effects of Non-opioid Compound, DDD-028, in the Rat Model of Paclitaxel-Induced Neuropathy
L Micheli, R Rajagopalan, E Lucarini, A Toti, C Parisio… - …, 2021 - Springer
【2】Dexpramipexole blocks Nav1. 8 sodium channels and provides analgesia in multiple nociceptive and neuropathic pain models
M Urru, M Muzzi, E Coppi, G Ranieri, D Buonvicino… - Pain, 2020 - journals.lww.com
【3】The Anti-Arthritic Efficacy of Khellin Loaded in Ascorbyl Decanoate Nanovesicles after an Intra-Articular Administration
G Vanti, L Di Cesare Mannelli, L Micheli, L Cinci… - Pharmaceutics, 2021 - mdpi.com
【4】Key role of gut microbiota in anhedonia-like phenotype in rodents with neuropathic pain
C Yang, X Fang, G Zhan, N Huang, S Li, J Bi… - Translational …, 2019 - nature.com
【5】Ameliorative effect of imperatorin in chemically induced fibromyalgia: role of NMDA/NFkB mediated downstream signaling
A Kaur, L Singh, N Singh, MS Bhatti, R Bhatti - Biochemical pharmacology, 2019 - Elsevier




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文献和实验[HTML] Fine‐touch pressure thresholds in the adult penis
ML Sorrells, JL Snyder, MD Reiss, C Eden… - BJU …, 2007 - Wiley Online Library
... The fine-touch sensitivity of 19 locations on the penis was measured using Semmes-Weinstein
monofilament touch-test sensory evaluators (North Coast Medical Supply, Morgan Hill, CA,
USA) [15–17] to create a penile sensitivity map (Figs 1 and 2). image ...
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[HTML] An experimental paradigm for the prediction of Post-Operative Pain (PPOP)
R Landau, JC Kraft, LY Flint, B Carvalho… - JoVE (Journal of …, 2010 - jove.com
... Pathway - Pain and Sensory Evaluation System, Medoc Advanced Medical Systems, B–kel 14L
Hot Tub, Boekel Scientific, 180g Touch-Test Sensory Evaluator, North Coast Medical, Morgan
Hill, California, Electronic von Frey, Bioseb, Cedex, France, References. ...
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Auditory grip force feedback in the treatment of writer's cramp
B Baur, W Fürholzer, C Marquardt… - Journal of Hand Therapy, 2009 - Elsevier
... The average age was 54.3 (±8.0) years. The mean duration of WC was 12.6 ± 16.9 years (range
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A Akbari, H Moodi, F Ghiasi… - Journal of …, 2007 - search.proquest.com
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... The static light touch test was performed using a Touch-Test Sensory Evaluator kit
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一、实验动物的标记 1. 染色法 适用于小动物,如兔子、大鼠、小鼠等。此方法是将化学药品涂在动物的被毛上,以不同颜色来区分动物。常用的化学药品有:3%~5%苦味酸溶液(黄色)、0.5%中性品红(红色)、2%硝酸银(咖啡色)。标记的原则是:先左后右,从上到下。如果动物编号是二位数或三位数,那相应的用二种或三种不同的颜色,不同颜色代表不同位数(个位、十位、百位),标记的原则同上。 2. 挂牌法 常用于大动物。写有编号的金属牌挂在动物的颈上来区分动物。对于猴、狗、猫等动物
和青蛙、蟾蜍常用氨基甲酸乙酯;对大鼠和小鼠常用硫喷妥钠或氨基甲酸乙酯麻醉。二、麻醉方法(一)全身麻醉:麻醉药经呼吸道吸入或静脉、肌肉注射,产生中枢神经系统抑制,呈现神志消失,全身不感疼痛,肌肉松弛和反射抑制等现象,这种方法称全身麻醉。其特点为抑制深浅与药物在血液内的浓度有关,当麻醉药从体内排出或在体内代谢破坏后,动物逐渐清醒,不留后遗症。1. 吸入麻醉法麻醉药以蒸气或气体状态经呼吸道吸入而产生麻醉者,称吸入麻醉,常用乙醚作麻醉药。吸入法对多数动物有良好的麻醉效果,其优点是易于调节麻醉的深度和较快
和青蛙、蟾蜍常用氨基甲酸乙酯;对大鼠和小鼠常用硫喷妥钠或氨基甲酸乙酯麻醉。二、麻醉方法(一)全身麻醉:麻醉药经呼吸道吸入或静脉、肌肉注射,产生中枢神经系统抑制,呈现神志消失,全身不感疼痛,肌肉松弛和反射抑制等现象,这种方法称全身麻醉。其特点为抑制深浅与药物在血液内的浓度有关,当麻醉药从体内排出或在体内代谢破坏后,动物逐渐清醒,不留后遗症。1. 吸入麻醉法 麻醉药以蒸气或气体状态经呼吸道吸入而产生麻醉者,称吸入麻醉,常用乙醚作麻醉药。吸入法对多数动物有良好的麻醉效果,其优点是易于调节麻醉的深度和较快
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