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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 国食药监械注册号:
无
- 保修期:
12个月
- 现货状态:
2周
- 供应商:
玉研仪器公司
- 规格:
敬请来电咨询
动物穿孔设备套装,大小鼠脑穿孔针主要用于冰冻脑切片的显微切割。

主要配件:
弹簧式尼龙推出器、操作手柄
两种主要型号:
5 punches & 3 punches

注:也可单独购买:Punch sizes are 0.25mm, 0.50mm, 0.75mm, 1.0mm, 1.25mm, 1.50mm,
1.75mm and 2.00mm (±5%, slight variation due to electro-polish etching to sharpen).
根据需要,可以选择 小动物皮肤打孔取样器
皮肤打孔器用于取芯、穿孔、以及从软的、薄的材料(如组织、皮肤、胶体、纸张等)上取下样品。
多用途,适用于对软体材质打孔使用,也可作为较软目标取样的的工具(比如:挖取凝胶),手柄由PP材料制造,器体中空:带有锋利周缘的打孔头,还有样品从打孔头内打出的弹射装置。
产品特点:
1.无菌
2.柱塞系统可弹出标本
3.按尺寸大小对应不同颜色,便于分辨
皮肤取样器的尖端为不锈钢材质,非常锋利。每个打孔器单独无菌包装,可保存5年。打孔直径有多种规格可选: 1.0mm, 1.5mm, 2.0mm, 3.0mm, 4.0mm, 5mm, 6mm, 7mm, 8mm, 10mm, 15mm, 20mm 打孔深度: 7mm建议一次性使用,非常适合组织处理和刑侦应用。在一些应用领域,打孔器可以在清洁后重复使用。
清洁方法:通过取芯空白滤纸清洁每个头; 用乙醇冲洗或用压缩空气喷雾除去干燥的样品。
产品主要规格:
15110-10 Miltex® 打孔器, ID 1.0mm, OD 1.27mm, 绿色/紫红色
15110-15 Miltex® 打孔器, ID 1.5mm, OD 1.75mm, 绿色/黄色
15110-20 Miltex® 打孔器, ID 2.0mm, OD 2.36mm, 绿色/褐色
15110-30 Miltex®打孔器, ID 3.0mm, OD 3.37mm, 绿色/粉红色
15110-40 Miltex® 打孔器, ID 4.0mm, OD 4.36mm, 绿色/蓝色

注:5mm,6mm,7mm,8mm,10mm,15mm,20mm的款式为进口的小批量订制产品,这几款没有标签(其中10mm,15mm,20mm三个型号为非灭菌包装,其他型号为灭菌包装),产品图片如下:

使用皮肤取样器对小鼠进行皮肤取样操作:

玉研仪器公众号

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文献和实验1.Lee JH, Han J-h, Woo JH, Jou I. 25-Hydroxycholesterol suppress IFN-γ-induced inflammation in microglia by disrupting lipid raft formation and caveolin-mediated signaling endosomes. Free Radical Biology and Medicine. 2022;179:252-265.
2.Moreira-de-Sá A, Gonçalves FQ, Lopes JP, et al. Adenosine A2A receptors format long-term depression and memory strategies in a mouse model of Angelman syndrome. Neurobiology of Disease. 2020;146:105137.
3.Martín‐Segura A, Ahmed T, Casadomé‐Perales Á, et al. Age‐associated cholesterol reduction triggers brain insulin resistance by facilitating ligand‐independent receptor activation and pathway desensitization. Aging Cell. 2019;18(3):e12932.
4.Gerace E, Polenzani L, Magnani M, et al. Antidepressant-induced increase in GluA2 expression does not translate in changes of AMPA receptor-mediated synaptic transmission at CA3/CA1 synapses in rats. Neuropharmacology. 2023;223:109307.
5.Di Menna L, Busceti CL, Ginerete RP, et al. The bacterial quorum sensing molecule, 2-heptyl-3-hydroxy-4-quinolone (PQS), inhibits signal transduction mechanisms in brain tissue and is behaviorally active in mice. Pharmacological Research. 2021;170:105691.
Chromatin Immunoprecipitation (ChIP)
. Make sure bead slurry is completely resuspended before adding to samples. 18. Centrifuge the samples at 3000 rpm for 2 min at 4°C. 19. Collect 15 µL from the supernatant of the control antibody sample as a 5
the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
most of the blood remaining in the solution. Add 10 brains into the Glass/Teflon Potter Elvehjem Tissue Grinder, add about 5 ml ice-cold sucrose buffer (with protease inhibitors, from Step 1) per brain and homogenize brains by 10 strokes (one stroke
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