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- Thermo scientific -fermentas
- ER0631
- 2025年10月05日
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| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS | |
| 10X Buffer G | 10X Buffer Tango™ | ||||
| ER0631 | 2500 u (10 u/µl) | 1.00 ml | 1.00 ml | ER0631 | |
| ER0633 | HC, 12500 u (50 u/µl) | 2 x 1.00 ml | 1.00 ml | ER0633 | |
| ER0635 | 5000 u (10 u/µl) | 2 x 1.00 ml | 1.00 ml | ER0635 | |
| ER0637 | 1000 u (10 u/µl) | 1.00 ml | 1.00 ml | ||
| Recommended buffer for 100% activity | Optimal temperature | Enzyme activity in Fermentas buffers, % | Tango™ buffer for double digestion | |||||
|---|---|---|---|---|---|---|---|---|
| B (blue) 1X |
G (green) 1X |
O (orange) 1X |
R (red) 1X |
Tango™ (yellow) 1X / 2X |
||||
| G | 37°C | 50-100* | 100 | 20-50 | 50-100 | 20-50* | 20-50* | 1X* or 2X* |
Lambda DNA
0.7%琼脂糖
15 切割位点
10 mM Tris-HCl (pH 7.5, 37°C), 10 mM MgCl2 , 50 mM NaCl, 0.1 mg/ml BSA。
37°C酶切。
10 mM Tris-HCl (pH 7.4, 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA和50% (v/v)甘油。
备注
Dcm: 无重叠 – 不影响。
CpG: 无重叠 – 不影响。
EcoKI: 无重叠 – 不影响。
EcoBI: 可能重叠 – 影响不确定。
| bp from the recognition site to fragment end | ||||
|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 |
| 50-100 | ||||
| Lambda | ΦX174 | M13mp18/19 | pBR322 | puc18/19 | pUC57 |
|---|---|---|---|---|---|
| 15 | 0 | 3 | 1 | 2 | 2 |
| pTZ19R/U | pTZ57R | pBluescriptIIKS(-/+) | pBluescriptIISK(-/+) | pACYC177 | pACYC184 |
| 2 | 2 | 2 | 2 | 0 | 2 |
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文献和实验Determining the Direction of Replication Fork Movement
, with the enzyme of choice in various brands of agarose. The enzymes that cut to completion in Beckman LE agarose are AvaI, Bam HI, BglII, EcoRV, NcoI, PstI, PvuII, SacII, SnaBI, SpeI, StuI, XbaI and XhoI. (There are likely to be many others that also cut
Mesoplasma florum:Transposome construction
Transposome DNA construction from plasmid cutting Cut the transposome out of the containing plasmid with PvuII enzyme, which cuts at the correct location at the mosaic end. Cut also with an enzyme which produces shorter
Quantification of mRNA Levels Using Ribonuclease Protection Assay
ends (shown by the gray color). ( C ) To prepare synthetic NT-3 mRNA, the plasmid is cut with PvuII , which cuts twice in the plasmid backbone. The presence of the vector-derived PvuII fragment will not interfere
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