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文献和实验Human In-Cell scFv Library from Infiltrating B Cell
to the study of the antibody repertoire formed in vivo during, for example, autoimmune diseases. We describe here, the use of the in-cell PCR together with Cre-recombination applied to human B cells to obtain in situ pairing of the variable (V
the beadbound cells with a magnet. Discard the bead-bound cells and use the remaining untouched, enriched cell population for further flow sorting into the DC subpopulation(s) of interest 实验试剂 Magnet: (Dynal® MPC™), MPC-L for 1–5 ml
Expression Cloning of Human B Cell Immunoglobulins
characterization of the human antibody repertoire on a single cell level through the generation of recombinant monoclonal antibodies from single primary human B cells of defined origin. This protocol offers a detailed description of the method starting
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