- ￥456
- hyclone
- 美国
- SH30529.FS
- 2025年12月16日
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- 供应商：
hyclone
- CAS号：
SH30529.FS
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文献和实验Purification of Endotoxin Free mAbs
Sulphate Precipitation Prepare saturated (NH4)2SO4, using Analar Grade reagent, 1kg per litre endotoxin free water, and autoclave in NaOH treated glass bottles. Store at 4 deg C. Mix supernatant above excess crystals before use to ensure
Purification of Plasmid from 50 ml-culture
2. Spin the bacterial culture at 6,000 rpm for 10 min at 4 C. Discard the supernatant. 3. (0ptional) Suspend the cell in about 20 ml of deionized water. Spin again. Discard the supernatant. Remove all of the supernatant fluid using pipetman.
have been described (1 -4 ), but in our experience, it is much better to avoid it from the beginning by scrupulous control of raw materials and use of good aseptic technique. To grow, bacteria need water; therefore, the most likely sources of endotoxin are water
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