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- 详细信息
- 文献和实验
- 技术资料
- 库存:
充足
- 供应商:
hyclone
- CAS号:
SV30079.01
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文献和实验Method: Removal of Yeast Contamination from Lymphoblast Cultures
only antibiotic and not the antimycotic. Solutions: Wash media: (1 liter) To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add: 10.0 ml 2.5 M (100X) Hepes buffer 1.2 ml 50 mg/ml gentamicin reagent Filter sterilize
Cell Culture Media and Solutions
165.0 ml fetal bovine serum, heat inactivated 12.0 ml 200mM (100X) L-glutamine 12.0 ml antimycotic/antibiotic (100X), liquid, Gibco, Cat. No. 600-5240AG Filter sterilize through a 0.22 � cellulose acetate filter and store
to 7.4 with 1 N NaOH or 1 M HCl. Dubelcco Modified Eagle Medium (Complete Media): DMEM (1x liquid tissue culture medium with 4.5 g/l glucose) 70 % 500 ml NCTC-135 medium 10% 50 ml Fetal bovine serum 20 % 100 ml Antibiotic/antimycotic 1% 5 ml
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