- ￥1500
- Kapa biosystems
- 美国
- kk5512
- 2025年12月20日
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- 英文名：
kk5512
- 库存：
充足
- 供应商：
Kapa biosystems
- 规格：
250 units
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文献和实验【精华】vol 687 Chapter 4 巢式PCR侧翼序列测定实验
,in which case, proceed to the next step. 5. Use 0.5 ml of product from the preceding step to template anested 50 ml PfuTurbo?hot start PCR in (see Note 13) 1×reaction buffer including 300 mM dNTPs, 3.75 U PfuTurbo® DNA Polymerase, and 250 nM GSP2 and GSP
Microscale Hot Borate RNA Extraction from Cotton Tissue--从棉花组织中提取微量RNA
℃. Alternatively: To assess the quality of the DNA, prepare a 1% agarose gel (0.30 g agarose in 30 mL 1X TAE buffer). Load 1 µL of each RNAsample into each well after mixing the aliquot with fast orange tracking dye. Run a ladder in one lane. The gel
; lower for plasmid / purified DNA / virus DNA target)Buffer: use proprietary or home-made 10x rxn mix; eg: Cetus, Promega. This should contain: minimum of 1.5mM Mg2+, usually some detergent, perhaps some gelatin or BSA. Promega now supply 25mM MgCl
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