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文献和实验oligonucleotide primer 1 50 μM oligonucleotide primer 2 5 unit/μl Taq Polymerase template DNA (1 μg genomic DNA, 0.1-1 ng plasmid DNA) in 10 μl
inoculation of 3 ml of antibiotic containing media with the plasmid or cosmid-containing bacterial colony, and then increasing the culture volume to 50 ml, and then to 4 l. However, it was observed that recombinant cosmid DNA isolated from cell cultures grown
template DNA 2 μl Bst reaction buffer 2 μl Bst nucleotide extension mix 1 μl oligonucleotide primer (2.5 ng/μl) 0.5-1 μl [alpha]-32-P-dATP or [alpha]-35-S-dATP 1 μl diluted Bst polymerase (0.1 U/μl) q.s. sterile ddH2O 12 μl [alpha]-32-P
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