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- QIGEN
- 德国
- 69106
- 2025年10月31日
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文献和实验【共享】经验:The RNeasy Mini Kit from Qiagen
and subsequently eluted in aqueous solution. The RNeasy kit can purify total RNA from animal cells or tissues, bacteria and yeast and another similar kit is available for the extraction of total RNA from plants and filamentous fungi, the RNeasy Plant Mini
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
medium and discard. To the bacterial pellet add 250 u Solution I/RNase A. Resuspend cells completely by vortexing or pipetting up and down. Complete resuspension of cell pellet is vital for obtaining good yields. 4. Add 250 u Solution II and mix
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
with occasional mixing. Avoid vigorous mixing as this will shear chromosomal DNA and lower plasmid purity. (Store Solution II tightly capped when not in use.) 4. Add 250 ul ice-cold Buffer N3 and mix gently but throughly by inverting tube several times
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