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- 2025年12月28日
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文献和实验transcripts, but have also been of value, in, for example, analysis of nuclear pre-mRNA splicing (1 –3 ), and in the detection of low abundance RNA species (2 ). There are four steps involved in performing a primer extension assay. First, selection
Microfluidic Primer Extension Assay
. As a modification, the so-called RAKE assay was developed that combined the use of unlabeled RNA with on-chip enzymatic labeling by exonuclease cleavage and polymerase primer extension (RNA 12:187–191, 2006; Nature Methods 1:155–161, 2004; Genome Res 16:1289–1298
Primer Design（Manual and Automated Primer Design for SEQ and PHY Gaps）
General Primer General Primer Design Guidelines Note the 5’-3’ direction of the contig. Locate primers 100 to 200 bp from the feature. Pick primer from ≥ 2x high quality sequence coverage
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