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Custom miScript Primer Assay (100)
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文献和实验[分享]冷泉港实验室PCR Primer上关于real-time的一节
. The design of a real-time PCR assay has more parameters than conventional PCR. Computer software is available to aid the design of real time PCR. e.g. Primer Express, and Beacon Designer. These programs include all of the design parameters required
Perlegen Assay Design Protocol
Perlegen Assay Design Protocol Description: Long-range PCR assays were designed using Oligo primer design software (Molecular Biology Insights). Primers were selected to have similar stringency and to map
, and then diluted 4- fold with water. For each ligation reaction, two to threePCRs are run in order to generate > 40 µg of PCR products. Each PCR contains 10 µL of the diluted ligation reactions (25 ng of starting DNA) in 100 µL volumes containing 1.0 µM of primer
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