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文献和实验Isolation of micro-RNA (miRNA)
succinic acid) β-Mercaptoethanol (β-ME) (14.4 M) Glycogen (2 mg/ml) Diethylpyrocarbonate (DEPC) DEPC-treated water 75% (v/v) ethanol (prepared using DEPC-treated water) Dounce, micro-Dounce, or mechanical
printed on the side of the cuvette. Adjust the meter to read 100% transmittance, using the right knob on the front of the instrument. This adjusts the instrument to read the upper limit of the measurements and establishes that your blank
to blank out any residual currents. This adjusts the lower limit of measurements. It establishes that no light is equivalent to 0 transmittance or infinite absorbance. Insert a clean cuvette containing the blank into the holder. Be sure
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