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文献和实验Myosin Light Chain Preparation
Buffer A.Homogenizing Buffer: 28 liters: 50 mM KCl,20 mM Tris (pH 8.0),15 mM MCE, 0.2 mM MgAc2 (350 ml 4 M KCl,280 ml 2 M Tris,28 ml 15 M MCE,1.20 g MgAc2) Buffer B.Triton X-100 Buffer: 4 liters of Buffer A plus 40 ml X-100 (1% Triton final) Buffer C
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
1 High-Energy Nd:YAG Laser Mirror, 25.4 mm Diameter, 45°, 532 nm Newport 10Q20HE.2 4 SUPREMA Optical Mount, 1.0 inch diameter
Methylated CpG Island Amplification
and 2 volumes 100% ETOH. Store at �70° C for 1 hour and centrifuge 30 min. at >10,000 g. Pour the ETOH out, air dry the pellets. Resuspend in 10-20 m l TE and determine DNA concentration using a spectro-photometer. 2.1.2 Ligation of Adapter
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