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文献和实验The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
Western Blotting with Monoclonal Ant
the enzyme conjugate anti-mouse IgG:HRPO (Catalog# M15345) 1:2000 in wash buffer containing 5% non-fat dry milk (or 1% BSA for phosphotyrosine antibodies). 3.Decant the wash buffer, add the diluted enzyme conjugate and incubate with agitation for 30 minutes
Western Blotting with Monoclonal Antibodies
for 30 minutes with agitation, changing the wash buffer every 3? minutes. Dilute the enzyme conjugate anti-mouse IgG:HRPO (Catalog# M15345) 1:2000 in wash buffer containing 5% non-fat dry milk (or 1% BSA for phosphotyrosine antibodies).
