正常人食管鳞状上皮细胞,Het-1A细胞基本信息
资源编号 hzCC288593
资源名称 Het-1A
种属 正常人食管鳞状上皮细胞
类型 epithelial; SV40 large T antigen transfected
形态 epithelial
提供形式 P1-2代冻存管
致病类型 normal
安全等级 2
模式菌株 未知
应用领域 The cell line may be useful for investigating the action of putative esophageal carcinogens.
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正常人食管鳞状上皮细胞,Het-1A细胞培养方法
培养基 90%高糖DMEM+10%FBS
生长条件 95%空气+5%二氧化碳 37摄氏度
生长特性 贴壁
存储条件 50%高糖DMEM+40%FBS+10%DMSO 液氮
正常人食管鳞状上皮细胞,Het-1A细胞详细说明
Age 25 years
Gender male
Ethnicity Black
Karyotype hypodiploid (34-40 chromosomes) RefStoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51 365-371, 1991. PubMed 1703038
Derivation The HET-1A cell line was derived in 1986 from human esophageal autopsy tissue by transfection with plasmid pRSV-T consisting of the RSV-LTR promoter and the sequence encoding the simian virus 40 large T-antigen.
Clinical Data 25 years
Black
male
Antigen Expression SV40 T antigenRefStoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51 365-371, 1991. PubMed 1703038RefTolleson WH, et al. The mycotoxin fumonisin induces apoptosis in cultured human cells and in livers and kidneys of rats. Adv. Exp. Med. Biol. 392 237-250, 1996. PubMed 8850621
Genes Expressed cytokeratin RefStoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51 365-371, 1991. PubMed 1703038
Cellular Products cytokeratinRefStoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51 365-371, 1991. PubMed 1703038
Tumorigenic No
Effects No, nontumorigenic in athymic, nude mice for more than 12 months.RefStoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51 365-371, 1991. PubMed 1703038
STR Profile Amelogenin X,Y
CSF1PO 10,12
D13S317 11
D16S539 9,11
D5S818 11,12
D7S820 9
THO1 7
TPOX 11
vWA 16
Year of Origin 1986
Restrictions The cells are distributed for research purposes only. The National Cancer Institute (NCI) releases the line subject to the following 1.) The cells or their products must not be distributed to third parties. Neither the cell line nor products derived from it may be sold or used for commercial purposes. 2.) Any commercial requests or proposed commercial use of these cells must first be negotiated with the Office of Technology Transfer, NIH, 6011 Executive Blvd., Room 325, Rockville, MD 20852; Telephone (301) 496-7057; FAX (301) 402-0220
描述 The line has undergone more than 250 population doublings. Growth factor studies have shown that HET-1A is stimulated by calcium and inhibited by fetal bovine serum, transforming growth factor-beta 1, and transforming growth factor-beta 2. Fumonisin B1 produces growth inhibition and increased apoptosis in HET-1A cells. The synthetic retinoid CD437 (6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalene carboxylic acid (AHPN/CD437)0 induces apoptosis of esophageal squamous HET-1A cells through the caspase-3-dependent pathway.正常人食管鳞状上皮细胞,Het-1A细胞HZC3697 小鼠背脊髓神经元 MN-dsc]
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