- ￥5000
- GeneTex
- 美国
- GTX77401
- 2025年07月08日
- WB, ELISA, IHC
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Rabbits were immunized with HA-tag conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and the antibodies were conjugated
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
- 克隆性：
Polyclonal
- 标记物：
Biotin
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX77401
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ELISA, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
HA tag
- 抗体英文名：
HA tag antibody (Biotin)
- 抗体名：
HA tag 抗体 (Biotin)
- 规格：
100 μg
ELISA analysis of immunogen using GTX77401 HA tag antibody (Biotin).
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文献和实验后将其移除。 纯化原理 Strep标签纯化蛋白质的技术原理，是利用自然界中最强的非共价作用之一：生物素(biotin)和链霉亲和素(streptavidin)之间的相互作用。将链霉亲和素改进后，就得到了非常稳定的Strep-Tactin®。Strep-Tactin®同样也是一种蛋白质，能高效且可逆的结合短肽标签Strep-tag®，而且它保留了结合生物素的能力。当生物素加入时，会作为特异性的竞争剂，用于洗脱Strep-tag®标签蛋白。 标签-配体 搭配Strep标签进行蛋白纯化的配体有2种
Protocol for anti-HA antibody Western Blotting
) 5) Incubate in primary antibody solution: I use PBS with 0.1% TWEEN-20 plus 5% BSA as the basic solution, but you can use TBS too. I dilute the HA antibody 1:5000, just to be sure you will see a signal. I haven't EVER seen appreciable background. I've
The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots against yeast protein
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