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- 文献和实验
- 技术资料
- 保存条件：
请参考说明书
- 保质期：
请参考说明书
- 英文名：
ELISA Plate Coating Solution - 10X Concentrate
- 库存：
大量
- 供应商：
欣博盛生物科技有限公司
- 规格：
1000 mL
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文献和实验7.4) with detergent such as 0.05% (v/v) Tween20 (TBST). Antibody solution: primary and secondary antibody should be diluted in 1x blocking solution to prevent nonspecific bindings. Coating antigen to microplate 1. Dilute the antigen to a final
DETECTION OF RECOMBINANT PROTEINS BY ELISA
volume with carbonate coating buffer (pH 9.6) to coat the ELISA plate. Two plates can be coated with the 1 times the volume, 4 plates with 2 times the volume. Label 96 well Costar (#2797) plates as needed. (See attached example of a 96 well plate
of 0.5 – 8 µg/ml in Coating Buffer and transfer 100 µl to each well of a high affinity, protein-binding ELISA plate. 2) Seal plate to prevent evaporation. Incubate at 4°C overnight. 2. Block the Plate: 1) Bring the plate
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