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细胞污染识别与处理全攻略:5 种常见类型+关键误区Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
请参考说明书
- 保质期:
请参考说明书
- 英文名:
Sircol™ Bovine Collagen Reference Standard (0.5 mg/ml)
- 库存:
大量
- 供应商:
欣博盛
- 规格:
3x 5 ml
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文献和实验鼠尾制备胶原蛋白-Making collagen solution from lyophilized rat tail collagen
3, make a 10X solution but with the pH color indicator, and use HCl to adjust pH to 4.0, then dilute this solution w/ water to make the 0.1X DME solution.) To each bottle of lyophilized collagen, add 2.5 mLs 0.1X DME pH 4.0 and 1.75 uL of a 10
Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer
flasks at approximately 1.3x104 cells/cm2 in Dulbeccos Modified Eagles Medium (DMEM) with 10% Fetal Bovine Serum (FBS). Culture cells for two days in an incubator at 37 °C and 5% CO2 . 2) Make fluorescent g-actin stock solution
, change 2 x daily). 10) Centrifuge dialysed collagen at 30,000 x g for 1.5 hrs. 11) Remove supernatant and place in sterile flask. 12) Adjust the collagen I concentration to ˜2 mg/ml using 0.5 mM acetic acid at 4°C.
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