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文献和实验Methylated DNA Immunoprecipitation (MeDIP)
Introduction Methylation of cytosines can mediate epigenetic gene silencing and is the most prominent DNA modification in eukaryotes. MeDIP is an immunocapturing approach to enrich DNA that is methylated. The principle
PCR purification kit) purify DNA through Qiaquick column elute DNA into 50 µl H2 O use 0.5 - 1 µl per 25 µl PCR reaction: 1 x 95 °C, 2 min 21 x 95 °C, 30 sec; 60 °C, 30 sec; 72 °C, 1 min 1 x 72
Dynabeads® Co-Immunoprecipitation Kit
Co-Immunoprecipitation Optimization below) from which the desired protein complex is isolated. The Extraction Buffer is made with 5 × IP supplied in the kit. The 5 × IP is supplied as 5 × concentrate and in sufficient quantity to allow for 40 coimmunoprecipitation
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