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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
, including the Genome Analyzer (Solexa/Illumina), 454 (Roche), and ABI-SOLiD (Applied Biosystems). This protocol describes sample preparation for sequencing of chromatin-immunoprecipitated DNA (ChIP-Seq) to analyze histone modification patterns using
Using ChIP-Seq Technology to Generate High-Resolution Profiles of Histone Modifications
immunoprecipitation (ChIP) assay allows investigators to characterize DNA–protein interactions in vivo. ChIP followed by hybridization to microarrays (ChIP-chip) or by high-throughput sequencing (ChIP-seq) are both powerful tools to identify genome-wide profiles
Increasing Sensitivity in Northern Analysis with RNA Probes
between single-stranded RNA and double-stranded DNA probes are not significant. ULTRAhyb is now a part of every NorthernMax™ Kit that Ambion sells. Using RNA probes in Northern analyses results in significantly greater sensitivity as compared to double-stranded
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