2×GC-Rich PCR Mix

An Economic PCR Enhancer for GC-Rich PCR Templates

  Since PCR is often problematic on GC-rich templates, we have evaluated different PCR enhancing additives and generated an Combinatorial Enhancer Solution (CES). We tested this solution on approx. 50 different primer pairs using

Minimum GC-Rich Sequences for Overlap Extension PCR and Primer Annealing

, the annealing sequences can be shortened. Here, we describe universal GC-rich annealing sequences useful for overlap extension PCR and simple in-frame addition of desired sequences.

GC-Rich Template Amplification by Inverse PCR: DNA Polymerase and Solvent Effects

The amplification of GC-rich templates by any PCR method is usually a difficult task and despite the development of modified methods and conditions, this type of amplification still remains a specific case approach. Problems usually observed